FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48465921
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA642660.13260038945716104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG152200.012.98072245
ATAGGGG158650.012.74540938
GTTGTCG28000.012.69432526
GGCATAG156350.012.68874735
GGGAGGT250150.011.7151023
GTCACTC221650.011.5460578
GGAGGTC241000.011.2560254
GAGCTTA180300.011.21836144
ACTCTCC240600.011.02178211
CATAGGG180750.010.92540937
GGTCACT237550.010.8302037
AGGTCAC257350.010.5196096
TGGCATA191550.010.47446134
GCATAGG190300.010.47182336
AGGGATC136600.010.3725982
CACCGAA330950.010.36875145
TTCTATA348350.010.168632
GCACCGA330450.010.13124144
GAGGTCA272050.010.0318215
TCTATAG345200.09.9813713
Produced by FastQC (version 0.11.5)