FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50852057
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT852670.167676599591635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT166450.024.37159245
TTCGACG23400.022.5956046
AGACCGC200950.020.9935174
CAGACCG211750.020.0927813
ACCGCGT222400.019.0193216
AGAACGA214750.019.01583544
AATACGG226150.018.60445632
GACCGCG227050.018.5802575
ATACGGA227100.018.5662633
TCGACGA29200.018.4156677
CCGCGTT230100.018.3633257
TACGGAG230600.018.31373634
CGCGTTC232550.018.1795378
CGGTATA39800.017.92043720
GTCCGTT36450.016.9895111
CAATACG251550.016.81533431
TTCGGCG92450.016.69510744
GCGTTCT265400.016.098919
AAGAACG263000.016.05759443
ACGGAGA263450.016.02162635
Produced by FastQC (version 0.11.5)