FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44882981
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1077800.2401355649706066No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT586080.13057956199477927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT362500.025.0706791
TCACGCT391400.023.363588
CCGGCTT406900.023.2888362
GAAAACG342200.022.3358220
TTCACGC414950.022.2543377
AACGCTA409950.021.85219423
ACGCTAC410800.021.79054524
CGCTACT105100.021.43211725
CGGCTTC459150.021.1266043
ACGCTCA444600.021.0182610
AAAACGC429200.020.93450521
CACGCTC438400.020.8536879
CGCTACC323500.020.7358325
AAACGCT438950.020.47513422
GTTTTCG456850.019.4731145
CGTGGTT468450.019.01201841
TCGTGGT483950.018.49156640
AGAAAAC420450.018.39680919
GGCTTCA554350.017.7946764
GTTAGTT162000.017.58107844
Produced by FastQC (version 0.11.5)