FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49789941
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1750160.3515087515367813No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG663890.13333817768532805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC314150.027.8302384
ACCGCGT317500.027.5904566
GACCGCG320750.027.2564545
CAGACCG323450.026.9257133
CCGCGTT330900.026.4663687
CGCGTTC339300.025.831038
TCAGACC360250.024.6623422
GAACGAT354050.024.52060545
GCGTTCT383100.022.9306079
AACGCTA162050.022.88270223
ACGCTAC162500.022.84771724
CTCAGAC427250.022.1954211
AGAACGA396400.022.00311944
AATACGG395050.021.9006732
ATACGGA399000.021.70663533
TACGGAG398750.021.65189634
CGCTACC171200.021.56850625
AAAACGC182700.020.41927521
CAATACG426200.020.40550631
CCCAATA433400.020.25354429
Produced by FastQC (version 0.11.5)