FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49789941
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA514520.10333814213597883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG168950.014.68908845
GGCATAG176250.014.17203335
GTTGTCG25700.013.2237926
ATAGGGG192250.013.21466838
GGGAGGT313100.013.1305873
GAGCTTA192650.013.08072644
ACTCTCC288350.013.02665611
GTCACTC289500.012.9745458
GGTCACT287600.012.9194897
TGGCATA200800.012.60716634
CATAGGG201950.012.4129737
ACCCGGC88200.012.3939765
TAGCGTA9300.012.34018337
GGAGGTC314150.012.1422214
AGGTCAC326050.011.7482576
GCGTAGG102050.011.422235536
GCATAGG219500.011.3794936
TCACCCG101650.011.3501343
GGCGTAG103150.011.27871435
TCACTCT341000.011.2591829
Produced by FastQC (version 0.11.5)