FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43722811
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1346470.3079559546160013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT253450.027.33130845
GACCGCG271800.025.543255
AGACCGC274450.025.4849414
ACCGCGT276400.025.1588386
TTCGACG25850.024.8905876
CAGACCG284050.024.805683
CCGCGTT285700.024.3476967
CGCGTTC289750.024.0073748
AGAACGA301300.023.09459944
TCAGACC323000.022.3089222
AATACGG313350.022.18786832
ATACGGA316600.021.96567733
TACGGAG320600.021.57163834
CAATACG333900.020.92399231
GCGTTCT336650.020.7563029
AAGAACG336600.020.71963743
TCGACGA32350.020.5152287
CTCAGAC379150.020.3463061
ACGGAGA350000.019.8810935
CCCAATA360400.019.54750629
Produced by FastQC (version 0.11.5)