FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42171496
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG476880.11308112000579729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG144050.016.2709945
GGCATAG153350.015.32137235
TAGCGTA14550.014.09958337
GAGCTTA168100.014.07856744
ACCCGGC96500.013.9423225
TGGCATA171900.013.89241534
CATAGGG171350.013.76180637
ATAGGGG181350.013.400269538
GTCACTC235350.013.3217728
GGTCACT243500.012.913327
GCATAGG185500.012.81093836
ATAGCGT16700.012.42097236
AGGTCAC263850.012.4199816
TCACCCG109500.012.4106033
ACTCTCC253400.012.3461411
ATAGCTC97450.012.28308439
ACGGACA104900.012.22373718
GAGGTCA276300.012.007025
CGGACAC108600.011.58061113
GGGAGGT319550.011.4507483
Produced by FastQC (version 0.11.5)