FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54703123
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1176370.21504622322933922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT235500.025.36475245
GACCGCG264350.022.3296555
ACCGCGT266250.022.227666
AGACCGC270250.022.0335124
CCGCGTT274950.021.5486567
CGCGTTC276950.021.3925348
AGAACGA286850.020.96549444
CAGACCG286150.020.9270763
AATACGG308800.019.09402732
ATACGGA311650.018.91221233
TACGGAG310150.018.90221834
TCAGACC329650.018.6773822
AAGAACG329900.018.17297643
GCGTTCT329600.018.104949
CAATACG330550.017.93294531
ACGGAGA346000.016.99193835
CTCAGAC405550.016.640731
CCCAATA371150.016.30597329
CGGAGAG365850.016.00222836
CCAATAC407900.015.01318330
Produced by FastQC (version 0.11.5)