FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44980622
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1765910.39259350393153747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT297900.027.78289245
AGACCGC332400.025.3119814
ACCGCGT332200.025.2183656
GACCGCG336800.024.874435
CAGACCG344100.024.7067683
CCGCGTT340250.024.6151077
CGCGTTC345750.024.2495428
AGAACGA346950.024.10895244
TCAGACC374650.022.974722
AATACGG380300.021.9593632
ATACGGA384500.021.69608133
GCGTTCT388150.021.675899
TACGGAG383650.021.64542234
CAATACG405600.020.66173631
CTCAGAC451050.020.482831
AAGAACG410550.020.4307243
ACGGAGA417300.019.97589335
CCCAATA431550.019.49611329
CGGAGAG433950.019.14717136
CCAATAC449450.018.8298630
Produced by FastQC (version 0.11.5)