FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44980622
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG634210.1409962716833929No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA463870.10312663084116533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG183800.016.81634145
GGCATAG199350.015.54814735
ATAGGGG211200.014.70391738
GAGCTTA222450.014.08681444
CATAGGG223900.013.86086237
TGGCATA226000.013.80445434
GGGAGGT324850.013.402123
GGAGGTC312150.013.3855454
GCATAGG236050.013.15718836
ACTCTCC312050.012.9478911
GTCACTC319450.012.725398
GGTCACT323900.012.5438587
ACCCGGC88600.012.0493455
ACGGACA87300.011.94313818
ATAGCTC82500.011.84740339
TAGGGGA344500.011.61023839
TGGGAGG291000.011.5471932
GTTGTCG23250.011.23411126
CACTCTC362550.011.20633410
GAGGTCA386100.011.0425945
Produced by FastQC (version 0.11.5)