FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53003548
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG41400.026.7910946
TCGACGA48800.022.8668167
CGACGAG48950.022.0613658
CGGTATA65350.020.86336720
GTTCGAC56700.019.3238585
ACCGCGT61050.016.8412536
AACGCTA133300.016.47459423
ACGCTAC135900.016.17610224
GACCGCG63800.016.1154885
AGACCGC66400.015.6214954
GTCCGTT44400.015.3718661
CAGTTCG82800.014.8915533
CCCGTAT35950.014.7243421
AGTTCGA79750.014.4735974
CCGCGTT71550.014.4012357
CGCTACT49200.014.36030725
TCACGCT166950.014.3518238
AAAACGC156500.014.24704821
ATGACGG92900.014.2398816
CAGACCG76400.014.0185583
Produced by FastQC (version 0.11.5)