FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53003548
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG161300.013.24111345
GGCATAG168400.012.47054135
ATAGGGG178150.012.25099738
CATAGGG185550.011.32674537
GGGAGGT250850.011.1927483
TAGCGTA8800.010.99524737
GTTGTCG33300.010.95391726
GCATAGG195050.010.82123736
GTCACTC238700.010.8147448
GAGCTTA197600.010.8009844
AACTCTA118500.010.56749545
GGAGGTC253850.010.5633894
GGTCACT243150.010.5151597
TGGCATA205550.010.27144434
ACCCGGC75000.09.9855065
AGGTCAC272450.09.8303686
GAGGTCA279700.09.8177135
ACTCTCC266300.09.69357511
TAATCGG46400.09.5995624
TGTCGTT40000.09.457911528
Produced by FastQC (version 0.11.5)