FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65012709
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2901330.4462712052192749No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG888740.1367025022753628No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA810920.12473253498788983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT515450.027.76126945
AGAACGA628450.022.85901644
AGACCGC639350.022.4221534
ACCGCGT640350.022.2259396
GACCGCG642800.022.1267595
CAGACCG654250.021.9252723
CCGCGTT660600.021.5476327
CGCGTTC661550.021.5239078
TCAGACC710500.020.627682
AATACGG717050.019.88881932
GCGTTCT721100.019.8212959
TACGGAG717450.019.7867934
ATACGGA722500.019.7387933
AAGAACG731450.019.6116943
CTCAGAC804800.019.2138181
CAATACG755900.018.95590631
ACGGAGA759950.018.71870435
CCCAATA794800.018.20930329
CGGAGAG781750.018.03843136
CGTTCTC817000.017.86641510
Produced by FastQC (version 0.11.5)