FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58615213
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG586510.10006105411576342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG190550.015.34082245
ATAGGGG208700.013.962131538
GGCATAG212350.013.71005535
CATAGGG231700.012.65539837
GTCACTC327500.012.5980188
GAGCTTA234250.012.59551944
GGTCACT330100.012.29443557
GGGAGGT358350.012.0723843
TGGCATA245150.011.96432834
GCATAGG245750.011.91335736
ACTCTCC355750.011.63627811
GGAGGTC377400.011.4332234
AGGTCAC382500.011.0329226
GAGGTCA408400.010.5759285
TATCCTA109500.010.1587895
AGCATAG113100.010.03131845
AGGGATC169950.09.9576672
TAGCGTA8850.09.91696537
TAGGGGA408500.09.90190439
TAGGCTA105650.09.74133110
Produced by FastQC (version 0.11.5)