FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64870860
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG205450.015.23052645
GGCATAG211500.014.79577635
ATAGGGG229300.013.98143238
GTCACTC332050.013.3903428
CATAGGG235550.013.32371637
GAGCTTA240000.013.18135744
GGGAGGT374500.013.0886963
GGAGGTC354200.012.8889414
GGTCACT343450.012.8541597
ACTCTCC352450.012.62764111
GCATAGG254000.012.39136536
TGGCATA254150.012.38781534
TAGCGTA11900.012.29736637
AGGTCAC379200.011.9864126
GAGGTCA391400.011.8480145
ACCCGGC111900.011.8031095
ACGGACA116650.010.98912818
GCGTAGG111050.010.8260136
CGTAGGG112600.010.77695637
GGCGTAG113850.010.67810235
Produced by FastQC (version 0.11.5)