FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50157262
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG20950.018.2525626
AACGCTA127850.017.7546723
ACGCTAC130000.017.46218324
CGCTACC135600.016.67466525
AAAACGC147250.015.61177821
GTCCGTT37250.015.5936841
TCACGCT159700.015.2812278
CGAACGA30600.014.85130732
ATGCGCG32000.014.49283944
TACGGTA68300.014.40445944
ACGCTCA175050.014.40379310
TGCGCGT32550.014.26279545
GAAAACG161800.014.24958120
TCGACGA28950.014.1410117
CCTTACG70650.013.98899241
CCCGTAT33400.013.8859641
ACGGTAC70900.013.8271145
GCCGGCT193100.013.781311
GTTAGTT119750.013.78049144
AAACGCT166400.013.7766322
Produced by FastQC (version 0.11.5)