FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54545360
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG545610.10002867338303387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG25100.021.0653726
AGACCGC117000.019.422764
GACCGCG117550.019.1596015
ACCGCGT118800.018.9201286
CCCGGTT68950.018.5366171
CAGACCG127450.018.0597633
GCCGGCT251850.018.0478651
TCACGCT282800.017.113398
GAAAACG181700.016.38301720
CCGGCTT324000.015.9792282
GTCCGTT42500.015.7777011
GCACTAC101350.015.76237125
TCGACGA36000.015.5622317
ACGCTCA331750.015.232594510
CACGCTC321050.015.1445759
TTCACGC323300.015.1157297
TCGGCGC89600.015.06699445
ACGGTAC83400.014.35254845
TTCGGCG94650.014.26310244
CGGCTTC376050.014.2518873
Produced by FastQC (version 0.11.5)