FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54545360
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA814580.14933992552253755No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG719730.13195072871459645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG221400.016.28046845
GGCATAG234900.015.25812635
ATAGGGG248450.014.48936838
GAGCTTA255450.014.06633744
TGGCATA259900.013.85103234
ACTCTCC371750.013.54582311
GGAGGTC377250.013.5215274
GAGGTCA380250.013.4917755
GGGAGGT407750.013.3930433
GGTCACT374150.013.2719347
AGGTCAC382300.013.2712526
GTCACTC374900.013.1795228
CATAGGG276700.013.00192737
TCACTCT396950.012.6004619
GCATAGG295000.012.24875536
CACTCTC403300.012.15690210
GTTGTCG27400.011.57809326
GCGTTCG31250.011.30461815
CCGCGTT33300.011.28440113
CGCGTTC32700.011.21620914
Produced by FastQC (version 0.11.5)