FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54449549
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG142750.014.30715845
ATAGGGG155800.013.41470638
GGCATAG155050.013.14327335
CATAGGG171100.012.21299337
GCATAGG174100.011.89575736
GAGCTTA179700.011.67734444
TGGCATA184200.011.30252934
GTCACTC235900.011.1939748
GGTCACT243000.010.7923547
ACCCGGC80350.010.7409925
GTTGTCG28350.010.70521726
GGGAGGT279400.010.6587853
ACTCTCC260600.010.48759311
GGAGGTC268450.010.2981674
TAGGGGA266250.09.71684539
AGGTCAC287950.09.5994066
GAGGTCA298650.09.3767985
TCACCCG94900.09.1656443
CGGACAC102450.08.82032513
TCACTCT316200.08.6138869
Produced by FastQC (version 0.11.5)