FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55208973
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG621200.11251794160344189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC194450.018.79087624
AACGCTA194350.018.77739123
CGCTACC198650.018.29165325
AGACCGC105550.018.2467234
AAAACGC220150.016.74033521
GACCGCG114950.016.5980175
ACCGCGT116600.016.4601146
CGTGGTT224300.016.31023641
CAGACCG120500.016.1322923
AAACGCT229300.016.11157822
CCCGTAT33450.016.016681
GAAAACG229650.015.93026820
GTTTTCG229900.015.81837445
GTCCGTT33600.015.275211
TCGTGGT241300.015.21709640
TCACGCT258300.014.7299458
TTCGACG15200.014.6545996
TCGTTAC21550.014.51514744
GCCGGCT262100.014.128321
CCGGCTT283800.013.7627962
Produced by FastQC (version 0.11.5)