FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55208973
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG22200.013.55763826
ATAGGGG131600.011.04487338
GGCATAG128250.010.69478435
GTTCTAT172500.010.6909561
AGCTTAG140600.010.20663945
TAGCGTA7100.09.5152937
TTCTATA341050.09.4514062
GGGAGGT247650.09.4150353
GAGCTTA151500.09.32659944
CATAGGG149650.09.25459737
CGTCAGT88800.09.2329911
GGAGGTC221950.09.1288134
ACCCGGC75250.09.0742585
TGGCATA154450.09.05975234
TTACGCG20100.08.94939135
GCATAGG155400.08.92631636
GCGTTCG16650.08.90299915
TGTCGTT35300.08.75133428
ATAGTGT367800.08.7313186
TCACCCG77750.08.696193
Produced by FastQC (version 0.11.5)