FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47637769
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG548100.11505576594067618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG172250.015.85393145
GGCATAG178200.015.19860135
ATAGGGG192100.014.25093738
GAGCTTA198350.013.90387244
GTCACTC250050.013.7799298
CATAGGG200200.013.52836737
GGTCACT258500.013.2771747
TGGCATA209100.012.93129534
GCATAGG210150.012.919991536
GGGAGGT299950.012.7468553
ACCCGGC83650.012.6718735
GGAGGTC287950.012.2544384
ACTCTCC283400.012.15833111
AGGTCAC296400.012.0956676
GAGGTCA307400.011.816415
ACGGACA89950.011.53280518
CCGGACA95300.010.98142512
TAGGGGA313200.010.895411539
CGGACAG95550.010.88021419
TCACTCT325150.010.7632969
Produced by FastQC (version 0.11.5)