FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40545970
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT448610.11064231537684262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29850.022.8368576
TCGACGA35050.019.5771667
CGGTATA50450.019.1316720
GTCCGTT31150.018.9464511
CGACGAG34300.018.759018
ACGCTAC87500.016.40737224
AACGCTA87300.016.36696623
GCACTAC70850.016.26192925
ATGCGCG26100.016.20523644
TGCGCGT26350.016.13686645
CTCCGAT46150.015.4241061
GTTAGTT144950.015.41235644
CGCTACC93550.015.20250725
CGATTAG33150.014.862734
TCACGCT104750.014.8624398
CTCGGTT74300.014.7343871
CCCGTAT26050.014.440871
AACGACG18600.014.39421816
ATGACGG63950.014.24848216
AAAACGC101800.014.23382521
Produced by FastQC (version 0.11.5)