FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46832035
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG971340.20740930860681156No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT705380.1506191221457705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA228700.025.33258623
ACGCTAC229900.025.21998424
CGCTACC235200.024.59428225
AGACCGC150150.023.8553714
AAAACGC247900.023.3977921
GCCGGCT235800.022.8947331
GAAAACG252350.022.78012320
CAGACCG158950.022.746983
TCACGCT260600.022.6805788
AAACGCT258800.022.47319222
CCGGCTT283500.021.3088342
TTCACGC285250.021.1228927
GACCGCG171850.020.8299925
GAACGAT172250.020.75549545
ACCGCGT174300.020.53726
CACGCTC299950.019.8926729
CGTGGTT299350.019.37635641
GTTTTCG279750.019.34253745
CCGCGTT185550.019.3041467
CGCGTTC186400.019.2281868
Produced by FastQC (version 0.11.5)