FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46832035
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG711600.15194727284432546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG213000.016.8054945
GGCATAG225000.016.13213335
ATAGGGG224750.016.02066838
GGTCACT314450.015.9476377
GGGAGGT328850.015.93522553
GTCACTC317700.015.8345418
GAGGTCA341900.015.0688575
AGGTCAC342200.015.0157026
GGAGGTC342300.014.8744884
GAGCTTA241700.014.8475244
ACTCTCC341600.014.58885211
TGGGAGG276950.013.869122
TGGCATA264650.013.80742834
CATAGGG269150.013.47817237
TCACTCT383600.013.2554139
GTTGTCG19550.012.65897826
GCATAGG291050.012.53308936
CACTCTC414000.012.0809910
GCTCTGT425300.011.88122122
TCTCCCC435700.011.46370713
Produced by FastQC (version 0.11.5)