FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48602853
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1493480.3072823729092611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT289650.025.858645
AGACCGC290300.025.8003274
ACCGCGT292400.025.3303346
GACCGCG292950.025.290465
CAGACCG303100.024.8592323
CCGCGTT304400.024.47227
CGCGTTC308050.024.1238048
AGAACGA328500.022.80747244
TCAGACC346950.022.4047262
AATACGG348300.021.38166432
GCGTTCT353050.021.1764229
TACGGAG350600.021.02320734
CAATACG360400.020.90727231
ATACGGA356950.020.8446133
AAGAACG359450.020.82488443
CTCAGAC408550.020.5859221
ACGGAGA379050.019.54619435
CCCAATA399050.019.12450229
CGGAGAG392350.018.71717636
CGTTCTC419900.018.35692610
Produced by FastQC (version 0.11.5)