FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48602853
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG116100.014.21228638
GGCATAG119000.013.80576135
AGCTTAG121350.013.35029745
CATAGGG126250.013.069674537
GCATAGG133700.012.29016436
TGGCATA145600.011.49728934
GAGCTTA142100.011.48027844
GGGAGGT221300.011.0100973
GGAGGTC213450.010.8141684
GTCACTC210850.010.6588658
GGTCACT211950.010.3170817
ACTCTCC211650.010.29968911
GTTGTCG29500.09.76164826
TAGGGGA227650.09.53239439
GGCGTAG57750.09.46975535
GCGTAGG57600.09.37896736
CGTAGGG58150.09.290812537
AGGTCAC248450.09.235976
AAGCCGG186100.09.18893945
AGGGATC112900.09.1469582
Produced by FastQC (version 0.11.5)