FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43916399
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA78400.020.40536723
ACGCTAC80700.019.82450324
CGCTACC86600.018.2937225
AAAACGC94500.017.23715221
TTCGACG20250.016.3314726
GTCCGTT34550.015.7106011
TCACGCT114250.015.1033128
GAAAACG107550.015.0616820
GTCGGTT45300.014.6174951
ACGCTCA120500.014.60006210
CCCGTAT26950.014.3745241
ACGGTAC60200.014.16437845
TACGGTA60800.014.09860744
AAACGCT117000.013.98054622
CCTTACG62000.013.78900341
ACGTTCG20900.013.7783346
TCGACGA25800.013.69037
GCCGTAT39600.013.2520961
GTCCGAT29600.013.1637071
TTACGGT66550.012.98187543
Produced by FastQC (version 0.11.5)