FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43916399
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC88600.012.0636055
GGCATAG93350.011.32631235
TCACCCG96050.011.0341853
AGCTTAG96950.010.88200145
CGGACAC93500.010.87873113
ACGGACA96700.010.68208218
ATAGCTC92550.010.64628939
ATAGGGG101800.010.56282738
CATAGGG102350.010.48407437
CACCCGG104700.010.3589954
CCGGACA103750.010.02086512
GCATAGG108900.09.77089436
TAGCTCT102050.09.76507140
CGGACAG106650.09.7065919
GCCCGGA118850.09.44775210
CACGGAC111950.09.3879117
ACACGGA111500.09.28408916
GACACGG113650.09.266726515
GTCACTC157600.09.1520648
GAGCTTA118200.09.09692644
Produced by FastQC (version 0.11.5)