FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53210018
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1154150.2169046437834319No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG630900.11856789824803292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC60300.016.088476
GTTGTCG57050.015.70951226
AGCTTAG229100.014.8522545
CACCCGG132400.014.59098151
GGAGGTC385400.014.3675024
ATAGGGG239850.014.29328738
TGTCGTT64650.013.88071928
GGGAGGT406050.013.8470153
GGCATAG244600.013.79936735
GTCGTTC66300.013.78924429
ACCCGGG138100.013.6634262
GAGCTTA260950.013.02612944
TATAGCT75800.012.7390237
TGGCATA272550.012.6356234
TCGTTCC75300.012.56082130
GAGGTCA453050.012.3603455
GCGTCGA31650.012.34603511
AATATAG94900.012.2844475
CGTTCCC76150.012.24254631
CAATATA90150.011.8110554
Produced by FastQC (version 0.11.5)