FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45708978
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG862240.18863690192329394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG43750.029.8280946
TCGACGA53350.024.7558217
GTTCGAC54200.023.9525685
CGGTATA75650.023.64459620
CGACGAG56700.023.0154048
GCCGGCT252650.021.6928831
CCGGCTT279450.021.2154872
AACGCTA266700.021.0991223
TTCACGC278300.021.0604767
ACGCTAC268000.020.97998424
TCACGCT278350.020.8869468
CGCTACC272150.020.5691225
ACGCTCA291000.019.99443810
AAAACGC289600.019.4617921
ATGACGG86900.019.18545516
CACGCTC309200.018.9121289
ACCGCGT44650.018.8462836
AAACGCT303950.018.61699122
CGGCTTC329650.018.5716723
GAAAACG302800.018.54651820
Produced by FastQC (version 0.11.5)