FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47317981
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG140250.014.64191845
ATAGGGG156300.013.309381538
GGCATAG153600.013.19525335
GAGCTTA159850.012.930510544
CATAGGG170450.011.90427937
GGGAGGT245100.011.7365833
GCATAGG173500.011.68179336
GTCACTC218950.011.6239388
TCAGACG84250.011.3823382
GGAGGTC243000.011.325164
TGGCATA184050.011.09676734
GGTCACT237300.010.91258057
ACTCTCC235900.010.82638411
AGGTCAC256200.010.6632736
GAGGTCA277550.010.0272515
CACCGAA158200.09.98506745
TAATCGG57300.09.939544
GCACCGA151250.09.85986144
TAGGGGA272350.09.79483339
GTAACGA57450.09.5250318
Produced by FastQC (version 0.11.5)