FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42307960
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG612330.1447316296980521No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT428410.10125990475551172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27850.025.9285876
TCGACGA33350.021.9215457
AGACCGC99250.021.5379854
CGGTATA45400.021.25579620
ACCGCGT100900.021.1579746
AACGCTA164050.020.98265523
GACCGCG102150.020.9484585
ACGCTAC165050.020.82897824
CGACGAG35650.020.1917558
CAGACCG107950.019.9689333
CGCTACC173400.019.73766125
CCGCGTT108950.019.5939797
AAAACGC181550.019.15662221
TCACGCT186850.019.0216268
CGCGTTC113200.018.778858
GCCGGCT178050.018.059081
GAAAACG193300.017.92110620
GAACGAT118600.017.60115845
AAACGCT200950.017.3526122
CACGCTC204750.017.325489
Produced by FastQC (version 0.11.5)