FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42307960
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN429270.1014631761966306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG119850.012.8196935
AGCTTAG122600.012.62420845
GTTGTCG25950.012.31006226
ACCCGGC77450.012.1162175
ATAGGGG130250.011.93324638
GGTCACT178500.011.6122567
TCACCCG89850.011.2677093
GGGAGGT223000.011.1883973
GTCACTC190500.011.1420478
TCAGACG68450.010.9443852
CATAGGG140450.010.922173537
CGGACAC88150.010.85292613
GAGCTTA143850.010.79064744
TGGCATA144400.010.70249234
GCATAGG144900.010.60322236
GGAGGTC206500.010.5698434
AGGTCAC211750.010.3709266
CACCCGG94100.010.3541674
ACTCTCC209750.010.17363811
CCGGACA96250.010.05652512
Produced by FastQC (version 0.11.5)