FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50188232
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG113450.012.1780235
GTTGTCG29400.011.70817826
ATAGGGG124750.011.41711738
ACGGACA90600.011.34940418
AGCTTAG123650.011.22124545
ATAGCTC88050.011.06476839
CATAGGG131350.010.82597237
GTCACTC188850.010.6849848
ACCCGGC102150.010.4180445
TCACCCG105500.010.3650933
GGTCACT189650.010.2122727
GCATAGG139250.010.16364836
TGTCGTT35600.010.11151228
CGGACAG97300.010.01263619
GAGCTTA144850.09.8631844
TAGCTCT102150.09.49304140
TGGCATA145850.09.47272134
CGGACAC109100.09.40495713
ATATAGC43850.09.3875966
TTGTCGT43700.09.16396327
Produced by FastQC (version 0.11.5)