FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53384568
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG174800.015.37038538
AGCTTAG174700.015.34754845
GGCATAG180500.014.89821335
GTCACTC265150.013.8427428
CATAGGG198900.013.55377337
GGGAGGT292800.013.3702343
GGAGGTC287100.013.3071114
GCATAGG206350.013.14059536
GAGCTTA208500.013.12902544
GGTCACT283900.012.8808867
ACTCTCC291950.012.87788611
TGGCATA217200.012.69197334
AGGTCAC315750.012.1212596
GAGGTCA322550.012.0078765
GTTGTCG33750.012.00056926
TAGGGGA324900.011.143846539
ATATAGC45750.011.1148646
CACTCTC344550.010.78295710
TCACTCT353000.010.7785969
GCGTAGG84700.010.68009436
Produced by FastQC (version 0.11.5)