FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52151054
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2279710.43713593976451554No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG795760.15258752009115675No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG754890.14475066985223348No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA674820.1293971930078345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT436800.026.4114245
TTCGACG37150.022.1942216
AGAACGA542400.021.6379144
ACCGCGT556450.021.4428656
AGACCGC557150.021.4282074
GACCGCG560300.021.271615
TCGACGA39350.020.9533757
CAGACCG570850.020.945473
CGGTATA52200.020.78789720
CCGCGTT575450.020.7270647
CGCGTTC579950.020.5739298
TCAGACC607350.019.7828852
AATACGG611000.019.19750432
ATACGGA616050.019.06201433
GCGTTCT630250.018.98549
TACGGAG631050.018.58756834
CAATACG641500.018.35830731
AAGAACG641650.018.31548143
CTCAGAC698150.018.2371541
ACGGAGA661500.017.75569235
Produced by FastQC (version 0.11.5)