FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52492845
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG150400.012.829452545
GGCATAG160500.012.08572835
ATAGGGG166250.011.73505838
GGGAGGT258800.011.0640313
GAGCTTA185700.010.49604644
GTCACTC251250.010.4015578
GGAGGTC259700.010.3493464
CATAGGG188350.010.33321837
TGGCATA194400.010.14064134
GGTCACT262750.09.7490797
GCATAGG200400.09.74602136
ACTCTCC272950.09.67412911
GTTGTCG25800.09.5067526
TCAGACG98750.09.1339932
ACCCGGC76100.08.9553465
TAGCGTA9550.08.95293237
TAGGGGA283100.08.950067539
AGGTCAC315750.08.66836
GAGGTCA338800.08.1921175
CAGACGT110700.08.0869493
Produced by FastQC (version 0.11.5)