FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41962771
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG492620.11739453526555718No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG477760.11385330106059965No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA457020.10891082478800077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27700.015.10676826
AGCTTAG163800.014.55727745
GGGAGGT214100.013.6538163
GGCATAG180950.013.20253235
ATAGGGG187250.013.19074238
GGAGGTC218650.013.1845994
GAGCTTA194250.012.36798444
TGGCATA206750.011.72922234
GGTCACT241450.011.7256457
GTCACTC242650.011.7233198
GAGGTCA250100.011.6797065
ACTCTCC251850.011.33089511
TAATCGG27500.011.2112794
CATAGGG215250.011.13001337
AGGTCAC267450.010.9558496
TGGGAGG235900.010.8369292
GCATAGG222700.010.82842536
CACCGAA241350.010.7838645
GCTAAGT40050.010.67636814
TAGCGTA6750.010.66437537
Produced by FastQC (version 0.11.5)