FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50088871
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG152650.014.00080545
GGCATAG161350.013.26039335
ACCCGGC99050.012.9566095
ATAGGGG177000.012.3928738
CATAGGG176850.012.1361737
GAGCTTA179500.012.00685944
GTCACTC235200.011.9395518
TAGCGTA10100.011.8057337
GGTCACT240400.011.6165147
GCATAGG184700.011.59617936
ACGGACA111350.011.45563118
CGGACAC112500.011.25939513
AGGTCAC257100.011.2216016
ACTCTCC256150.011.20799711
TGGCATA195000.011.16825134
ATAGCTC111750.011.03246839
TCACCCG120150.011.0185743
GGGAGGT302000.010.7952693
CCGGACA121000.010.7288912
CGGACAG119500.010.48608919
Produced by FastQC (version 0.11.5)