FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48063955
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1934780.4025428202901738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT304600.031.875645
AGAACGA364850.026.6231344
AGACCGC379750.025.7644544
ACCGCGT382600.025.5078666
GACCGCG384050.025.3881345
CAGACCG388000.025.3383623
CCGCGTT394550.024.7352947
CGCGTTC398800.024.466058
AATACGG410350.023.64867832
AAGAACG411500.023.63417243
ATACGGA382900.023.59006733
TACGGAG383600.023.53761134
GCGTTCT428350.022.8358849
TCAGACC436200.022.7911422
CAATACG426450.022.77951631
TTCGACG28850.022.063286
ACGGAGA413800.021.94220735
CCCAATA461800.021.16088929
CTCAGAC490250.021.1310231
CCAATAC471950.020.7913530
Produced by FastQC (version 0.11.5)