FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53568549
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1281080.23914778800523417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT286100.023.23864745
ACCGCGT297150.022.170166
AGACCGC301350.022.0824624
GACCGCG302500.021.7153385
CAGACCG312400.021.3735373
CCGCGTT310100.021.2443187
CGCGTTC314400.020.9609158
TCAGACC352500.019.7351282
TTCGACG24800.019.2269046
AGAACGA347800.019.18832444
AATACGG357850.018.46840732
ATACGGA361600.018.27677733
TACGGAG359500.018.20828834
GCGTTCT364300.018.176239
ACGTTCG34900.017.1427846
CTCAGAC467650.017.0871561
ACGGAGA390300.016.80548335
TCGGCGC135500.016.67712245
CAATACG409500.016.32050131
CCCAATA423750.015.98973929
Produced by FastQC (version 0.11.5)