FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53568549
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG709320.1324135174913922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC123900.015.8895815
AGCTTAG245800.014.95958745
TCACCCG136700.014.8600513
ACGGACA138850.014.04894218
CCGGACA142650.013.83572912
CGGACAG141550.013.74818619
CGGACAC140750.013.63869513
GTCACTC365000.013.5890688
GTTGTCG32300.013.51144826
GGCATAG272500.013.48964135
GGTCACT376250.013.2117687
CACCCGG158850.013.071854
ACTCTCC392000.012.77370711
GGGAGGT445100.012.6958893
ATAGCTC146900.012.63441439
TAGCGTA17750.012.5473437
GAGCTTA296000.012.49795344
GGAGGTC410150.012.48395254
ACACGGA157500.012.30117316
AGGTCAC418700.012.128676
Produced by FastQC (version 0.11.5)