FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47817862
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1459920.3053085058466228No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA817010.17085874730242018No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG624570.1306143716755885No Hit
CTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCC495280.10357635813997706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA246550.021.62694545
ATATAGC81600.021.4858596
GGAGGTC502050.020.7603154
GGGAGGT505450.020.4534153
GAGGTCA541950.019.1472995
CATGCAT162450.018.3269069
ATACATG165950.018.2410746
TATATAC122400.018.0191427
TGGGAGG471650.017.7285732
CTGGGAG511750.017.5145051
AATATAG109750.017.4333255
CAATATA107400.017.3550534
TACAATA109500.017.1064912
GTTGTCG92900.017.07224826
TATAGCT102800.017.0105137
AAACTCT324800.016.57576244
ATATATA113850.016.4868776
AAAACTC331100.016.36901943
GCTAAGT103900.016.00233814
AGGTCAC666350.015.6617436
Produced by FastQC (version 0.11.5)