FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54977207
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG614600.11179178309294613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG189500.015.68401345
GGCATAG196250.015.24772935
ATAGGGG206300.014.439507538
CATAGGG221100.013.58488937
GGTCACT292100.013.5492877
GTCACTC296750.013.3753358
GGGAGGT322050.013.2949833
GAGCTTA230150.013.12889844
GCATAGG232800.012.93112636
GGAGGTC324200.012.7348944
ACTCTCC314500.012.67824711
TGGCATA237350.012.66427834
TCAGACG110500.011.8300172
GAGGTCA359300.011.6224955
TGCCCGT192350.011.60333244
TAGCGTA13800.011.575582537
AGGTCAC356300.011.5370146
TAGGGGA364400.010.7123539
TCACTCT379200.010.7103999
ACCCGGC86750.010.5561015
Produced by FastQC (version 0.11.5)