FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44822848
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1097050.2447524084145657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT225450.025.4747076
AGACCGC227350.025.4694044
GAACGAT229150.025.27145445
CCGCGTT233200.024.5895127
GACCGCG237350.024.2444425
CGCGTTC239000.023.9833688
CAGACCG244900.023.7768523
TCAGACC272950.021.902232
AGAACGA266150.021.79267944
AATACGG267650.021.46609532
ATACGGA268000.021.40421133
TACGGAG268650.021.150234
GCGTTCT277100.020.7344749
CAATACG284400.020.39301731
CTCAGAC335600.019.4985661
AAGAACG298050.019.49797243
ACGGAGA293550.019.43236435
TTCGACG19200.018.9811466
CGGAGAG308050.018.4071636
CCCAATA321400.018.31162529
Produced by FastQC (version 0.11.5)