FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49279936
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA571560.11598229348349803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG136850.014.02163845
GGCATAG139100.013.56666935
ATAGGGG145550.013.15031938
CATAGGG155000.012.43620837
GTCACTC210050.012.3228478
GAGCTTA161150.011.93473144
TGGCATA163150.011.67609434
GTTGTCG23400.011.53903726
GGTCACT220850.011.5252717
GCATAGG166600.011.47577236
ACTCTCC227650.011.45992611
AGGTCAC238850.011.1928446
AGACTCA215050.011.0486044
TCAGACG94050.010.6207332
GGGAGGT268100.010.5814043
GAGGTCA261000.010.2770135
GGAGGTC267250.010.0018814
ATAGCTC73350.09.90427539
ACCCGGC80000.09.8167235
TCTATAG319300.09.8006123
Produced by FastQC (version 0.11.5)