FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50554925
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT509880.10085664255262963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG35350.026.2225846
CGGTATA55650.022.35772720
ACCGCGT119550.021.9252226
GACCGCG120700.021.6044835
TCGACGA44600.021.4902977
AGACCGC123400.021.36884
CCGCGTT128300.020.342257
CAGACCG133850.020.1207183
CGACGAG45550.020.1035948
CGCGTTC135650.019.2897958
GTTCGAC49050.018.8984385
GAACGAT162200.016.40973545
TCAGACC179500.015.7807982
AACGCTA147750.015.54769323
ACGCTAC148350.015.46964424
AATACGG176000.015.02098732
ATACGGA178700.014.83180533
ATGACGG79200.014.74381416
TACGGAG178550.014.74345534
CAGTTCG69800.014.7308723
Produced by FastQC (version 0.11.5)