FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50554925
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG137450.013.12802645
ATAGGGG143550.013.05826638
GGCATAG138900.013.02488535
GGGAGGT231300.011.6745893
GTCACTC212850.011.6269688
CATAGGG156900.011.545135537
GTTGTCG31550.011.26563826
GGTCACT216550.011.1477967
GGAGGTC230000.011.1221384
GCATAGG164600.011.0596536
AGGTCAC228700.011.0472296
GAGCTTA167100.011.04187644
TGGCATA175850.010.39043534
TAGCGTA7250.010.24240837
GAGGTCA251150.010.2391715
ACTCTCC241750.010.218411
ATATAGC51400.09.6731726
AACTCTA120500.09.20512545
ACCCGGC71650.09.1689255
TGTCGTT38350.09.0927628
Produced by FastQC (version 0.11.5)