FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42793920
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1380480.32258788164299973No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG519170.12131863591837347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG276350.024.3482635
AGACCGC279000.024.2298814
GAACGAT281000.024.1127945
ACCGCGT280250.024.0572536
CAGACCG287300.023.8670063
CCGCGTT288250.023.3583037
CGCGTTC293900.022.9016068
TTCGACG30950.022.4596376
TCAGACC331800.021.1411192
AGAACGA327500.020.77241944
GCGTTCT328000.020.6097419
AATACGG329450.020.5806332
ATACGGA332200.020.3903233
TACGGAG333800.020.19914434
TCGACGA35500.019.264127
CTCAGAC412100.019.0280111
CCCAATA368900.018.64680129
ACGGAGA365050.018.59356135
CGGTATA53500.018.58548520
CAATACG369600.018.4120231
Produced by FastQC (version 0.11.5)